Fitc-conjugated pac-1

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August undefined, 2024

WebDec 1, 2024 · The mono-conjugated FITC-insulin (22%) with unlabeled insulin prepared from 0.25:1 molar ratio (Fig. 3 A), tri-conjugated FITC-insulin (84%) with little amount of di-conjugate prepared from 4:1 molar ratio (Fig. 2 D) and the unlabeled insulin were chosen for the transport study. The percentage amount of mono-conjugate and tri-conjugate … WebClone HIT3a. Flow cytometric analysis of CD3 expression on human peripheral blood lymphocytes. Whole blood was stained with either FITC Mouse IgG2a, κ Isotype Control (Cat. No. 555573; dashed line histogram) or FITC Mouse Anti-Human CD3 (Cat. No. 561802/555539; solid line histogram). Erythrocytes were lysed with Lysing Buffer (Cat. …

FITC Mouse Anti-Human CD3 - BD Biosciences

WebConjugate: FITC. Order Details. View Available Conjugates. Catalog# & Conjugate Size Price; Alexa Fluor 350 NBP2-62201AF350: Alexa Fluor 405 NBP2-62201AF405 ... (PAC-1) [FITC] and receive a gift card or discount. Submit a Review. Bioinformatics. Gene Symbol: ITGA2B Popular Products ... Websupplied, and the antibody subclass for each FITC-conjugated antibody. The amount of antibody provided is sufficient for 25 immunostaining reactions using a 1 mL working solution per reaction. Antibody Concentration Amount Subclass Anti-His(C-term)-FITC 1 mg/mL 50 μl (25 reactions)† IgG 2b Anti-myc-FITC 1 mg/mL 50 μl (25 reactions)† IgG 1 jepang clock

PAC-1 antibody (FITC) anti PAC-1 antibody (FITC)

WebMutated KRAS proteins are often constitutively active, resulting in the dysregulated growth and transformation pathways that promote pancreatic cancer growth, survival, and metastasis. Proteintech’s KRAS antibody is a favorite among researchers worldwide, cited in nearly 100 publications. Immunohistochemical analysis of paraffin-embedded ... WebConjugated Antibodies for IF ... FITC Plus . HRP . PE . Clear Confirms. Conjugate. 0. Alexa Fluor® 488 Alexa Fluor® 568 ... The most efficient cooling rate is 1°C per 1 minute, which can become achieved of putting vials into a commercial cryofreezing container or with wrapping them in ampere thick layer of cotton wool and placed in a -80°C ... Web(340507) BD™ FITC Mouse Anti-Human PAC-1 - BD Biosciences - CiteAb. 156 citations – A FITC-conjugated mouse monoclonal antibody, raised against Proteasome assembly chaperone 1 (Human), supplied by BD Biosciences. ... Conjugate FITC Target Proteasome assembly chaperone 1 (Human) UniProt O95456 - PSMG1_HUMAN. Gene PSMG1 … pacific hard tube bending kit

Fluorescein Isothiocyanate - an overview ScienceDirect Topics

PAC-1 Antibody (4O21) SCBT - Santa Cruz Biotechnology

WebSpecifications. Name. Anti-PAC-1 Antibody [PAC-1] (FITC) Description. Mouse monoclonal [PAC-1] antibody to PAC-1 (FITC). Specificity. This antibody recognizes an extracellular activation-induced conformational epitope PAC-1 on CD41/CD61 complex (gpIIb/IIIa), also known as integrin alpha IIb beta 3, a receptor which mediates platelet aggregation ... WebFeb 16, 2024 · The blood platelets were distinguished based on the expression of CD61. For each sample, 10,000 CD61-positive objects (platelets) were acquired. For the assessment of P-selectin expression, samples were labeled with fluorescently conjugated monoclonal antibody PAC-1-FITC. Results are shown as the percentage of platelets binding PAC … pacific harbour villas fijiWebThe PAC-1 antibody is derived from the fusion of SP-2 mouse myeloma cells with splenic lymphocytes from BALB/c mice that were immunized with human platelets. The PAC-1 recognizes an epitope on the glycoprotein IIb/IIIa (gpIIb/IIIa, α IIb β 3) complex of activated platelets at or near the platelet fibrinogen receptor. jeparit and district nursing home

"WebApr 7, 2024 · And then, they were thoroughly washed, stained orderly with the vinculin antibody (1:500) overnight at 4 °C, the FITC-conjugated goat-anti-rabbit secondary antibody (1:5000) for 1 h, rhodamine-conjugated phalloidin (1:250) for 1 h, and DAPI (1:5000) for 5 min at room temperature. " - Fitc-conjugated pac-1

Fitc-conjugated pac-1

Standardization and reference ranges for whole blood platelet

WebConjugation: FITC: Tested applications: FACS, ICC, IF, IP: Immunogen: Human platelets: Target: PAC-1 (FITC). The mouse monoclonal antibody PAC-1 recognizes an activation-induced conformational epitope PAC-1 … WebCells were blocked with 1% BSA for 30 min and incubated for 60 min with 1:200 dilution of FITC-conjugated rabbit polyclonal anti-C1q antibody (Abcam, Cambridge, MA) and 0.4 μg/ml polyclonal, C-terminal specific rabbit anti-AQP4 antibody (Santa Cruz Biotechnology). Cells were incubated for 30 min with 5 μg/ml goat anti-rabbit IgG-conjugated ...

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WebThe binding of fibrinogen to the gpIIb/IIIa receptor is required for platelet aggregation. PAC-1 binds only to activated platelets and appears to be … WebUse RGDS with conjugated PAC-1 antibody NOTE: Some commercial preparations can include lipopolysaccharide (LPS), a potent platelet activator. FACS brand flow cytometer. Refer to the appropriate instrument user’s guide for detailed information. ... stain with PAC-1 FITC/CD62P PE/CD61 PerCP in the presence of RGDS to inhibit PAC-1 binding ...

WebThe PAC-1 Antibody FITC has been validated for the following applications: Flow Cytometry. Flow cytometry of PAC-1 in PHA-activated human peripheral blood. Sample was incubated with PAC-1 monoclonal antibody (Product # MA5-28564) conjugated to FITC. Weball FITC has dissolved. Label vial “20:1 FITC”. The solution should be freshly prepared before each conjugation and used within 5 minutes (See Application Note 6). The "20:1 FITC" solution is further used to prepare the "5:1" and "10:1" solutions. 5. Prepare the required dilution of FITC in 0.1 M carbonate-bicarbonate buffer as directed in ...

WebCatalog number: A13199. Achieve quick and reliable detection of early cell apoptosis with Annexin V stand-alone conjugates for apoptosis detection. Annexin V conjugates offer up to 100-fold difference in fluorescence signal intensity between apoptotic and non-apoptotic cells using flow cytometry. Contains 1 vial of annexin V, FITC conjugate. WebThe antibody was conjugated with FITC under optimum conditions, and unreacted FITC was removed. Store undiluted at 4°C and protected from prolonged exposure to light. ... a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 518-nm. FITC is designed to be excited by the Blue …

WebAug 1, 2003 · MFI of PAC-1-FITC was assessed in 5 whole blood samples immediately, 15, 30, and 60 min after antibody labelling with PAC-1 and CD42b MoAbs.

pacific hardwood orange caWebData on immunogenicity of adenovirus-vectored vaccine in chronic obstructive pulmonary disease (COPD) patients is limited. Therefore, we aimed to determine the humoral and cellular immune responses after homologous ChAdOx-1 vaccination in subjects with COPD. COPD subjects and age- and sex-matched healthy elderly receiving ChAdOx-1 … jepang world cupWebApr 4, 2024 · PAC-1 Antibody (4O21) is available as both the non-conjugated anti-PAC-1 antibody form, as well as multiple conjugated forms of anti-PAC-1 antibody, including agarose, HRP, PE, FITC and … pacific hardware window windersWebFluorescein Isothiocyanate (FITC)-conjugated phal- loidin was obtained from Invitrogen (Invitrogen, Carlsbad, CA, USA). Alexa 488-conjugated anti-rabbit anti-body and 4′,6-diamidino-2-phe- nylindole (DAPI) were obtained from Invitrogen. HPLC solvents were purchased from Fisher Scientific Ltd (Korea). General experimental procedures jeparit rainbow football clubWebPAC-1 Monoclonal Antibody (PAC-1), FITC, Invitrogen™-Mouse Monoclonal Antibody Shop PAC-1 Monoclonal Antibody ... PAC-1 Monoclonal antibody specifically detects PAC-1 in Human samples. It is validated for Flow Cytometry ... PAC-1: Classification: Monoclonal: Conjugate: FITC: Gene: EIF3EP1: Immunogen: Human platelets: Quantity: 100 Tests ... pacific harish industries limitedWebphore-conjugated monoclonal antibodies (mAbs): phycoerythrin (PE)-conjugated GPIb (CD42b, Clone HIP1), allophycocyanin (APC)-conjugated P-selectin (CD62P, Clone Psel.KO2.3) (both eBioscience, San Diego, CA, USA), and ﬂuorescein isothiocyanate (FITC)-conjugated PAC-1 (Becton Dickinson Bioscience, San Jose, CA, USA). jeparit railway stationWebPac 1 Fitc Conjugate, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more pacific harish ind ltd nashik mh